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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
06/12/2005 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JHAM, G. N.; SILVA, A. A. da; LIMA, E. R.; VIANA, P. |
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Afiliação: |
PAULO AFONSO VIANA, CNPMS. |
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Título: |
Identification (GC and GC-MS) of unsaturated acetates in Elasmopalpus lignosellus and their biological activity (GC-EAD and EAG). |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Journal of Separation Science, Weinheim, v. 28, n. 3, p. 281-285, 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
Two insect colonies of Elasmopalpus lignosellus were reared in our laboratory, the first being initiated from pupae obtained from a cornfield in the region of Sete Lagoas, Minas Gerais and the second from a cornfield in the region of Goiania, Goias. From the two colonies, two extracts were prepared from the pheromone glands of virgin E. lignosellus females. The extract obtained from the first colony was designated as extract 1 while the extract obtained from the second colony was designated as extract 2. Extract 1 was analysed by gas chromatography-mass spectrometry (GC-MS) with (Z)-9-hexadecenyl acetate [(Z)-9-HDA] and (Z)-11-hexadecenyl acetate [(Z)-11-HDA] being identified and confirmed by the formation of DMDS derivatives. In addition, a third acetate, which could be either (E)-8-hexadecenyl acetate [(E)-8-HDA] or (E)-9-hexadecenyl acetate [(E)-9-HDA] was detected by GC-MS. Extract 2 was analysed by gas chromatography (GC) and gas chromatography-electroannetography (GC-EAD) revealing the presence of (Z)-11-HDA and (Z)-9-TDA. In addition, the same compounds elicited a response with the E. lignosellus male antenna obtained from the second insect colony. Electroantennography (EAG) screening with the male E. lignosellus antenna (obtained from the second insect colony) was conducted with the 23 possible tetradecenyl acetates (TDA) and 22 hexadecenyl acetates (HDA) as standards. Out of the 23 TDA isomers evaluated, only (Z)-9-TDA elicited a response and out of the 22 HDA [(Z) and (E) isomers Delta 2 to Delta 13] evaluated only (Z)-11-HDA elicited a response. The acetate compositions of two extracts obtained from insects originating from the two states (Minas Gerais and Goias) of Brazil were different from one another as well as from that obtained from insects in Tifton, GA, USA. The bioactivity data (GC-EAD) of the extract 2 differed from those reported for the Tifton, GA, USA population. These data suggest polymorphism in relation to the insect populations found in Brazil and in the USA. The possibility of the existence of an E. lignosellus sub-species cannot be ruled out. MenosTwo insect colonies of Elasmopalpus lignosellus were reared in our laboratory, the first being initiated from pupae obtained from a cornfield in the region of Sete Lagoas, Minas Gerais and the second from a cornfield in the region of Goiania, Goias. From the two colonies, two extracts were prepared from the pheromone glands of virgin E. lignosellus females. The extract obtained from the first colony was designated as extract 1 while the extract obtained from the second colony was designated as extract 2. Extract 1 was analysed by gas chromatography-mass spectrometry (GC-MS) with (Z)-9-hexadecenyl acetate [(Z)-9-HDA] and (Z)-11-hexadecenyl acetate [(Z)-11-HDA] being identified and confirmed by the formation of DMDS derivatives. In addition, a third acetate, which could be either (E)-8-hexadecenyl acetate [(E)-8-HDA] or (E)-9-hexadecenyl acetate [(E)-9-HDA] was detected by GC-MS. Extract 2 was analysed by gas chromatography (GC) and gas chromatography-electroannetography (GC-EAD) revealing the presence of (Z)-11-HDA and (Z)-9-TDA. In addition, the same compounds elicited a response with the E. lignosellus male antenna obtained from the second insect colony. Electroantennography (EAG) screening with the male E. lignosellus antenna (obtained from the second insect colony) was conducted with the 23 possible tetradecenyl acetates (TDA) and 22 hexadecenyl acetates (HDA) as standards. Out of the 23 TDA isomers evaluated, only (Z)-9-TDA elicited a response and out of the 22 HDA [(Z) ... Mostrar Tudo |
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Thesagro: |
Inseto. |
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Thesaurus Nal: |
acetates. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02687naa a2200181 a 4500
001 1487835
005 2018-05-30
008 2005 bl uuuu u00u1 u #d
100 1 $aJHAM, G. N.
245 $aIdentification (GC and GC-MS) of unsaturated acetates in Elasmopalpus lignosellus and their biological activity (GC-EAD and EAG).$h[electronic resource]
260 $c2005
520 $aTwo insect colonies of Elasmopalpus lignosellus were reared in our laboratory, the first being initiated from pupae obtained from a cornfield in the region of Sete Lagoas, Minas Gerais and the second from a cornfield in the region of Goiania, Goias. From the two colonies, two extracts were prepared from the pheromone glands of virgin E. lignosellus females. The extract obtained from the first colony was designated as extract 1 while the extract obtained from the second colony was designated as extract 2. Extract 1 was analysed by gas chromatography-mass spectrometry (GC-MS) with (Z)-9-hexadecenyl acetate [(Z)-9-HDA] and (Z)-11-hexadecenyl acetate [(Z)-11-HDA] being identified and confirmed by the formation of DMDS derivatives. In addition, a third acetate, which could be either (E)-8-hexadecenyl acetate [(E)-8-HDA] or (E)-9-hexadecenyl acetate [(E)-9-HDA] was detected by GC-MS. Extract 2 was analysed by gas chromatography (GC) and gas chromatography-electroannetography (GC-EAD) revealing the presence of (Z)-11-HDA and (Z)-9-TDA. In addition, the same compounds elicited a response with the E. lignosellus male antenna obtained from the second insect colony. Electroantennography (EAG) screening with the male E. lignosellus antenna (obtained from the second insect colony) was conducted with the 23 possible tetradecenyl acetates (TDA) and 22 hexadecenyl acetates (HDA) as standards. Out of the 23 TDA isomers evaluated, only (Z)-9-TDA elicited a response and out of the 22 HDA [(Z) and (E) isomers Delta 2 to Delta 13] evaluated only (Z)-11-HDA elicited a response. The acetate compositions of two extracts obtained from insects originating from the two states (Minas Gerais and Goias) of Brazil were different from one another as well as from that obtained from insects in Tifton, GA, USA. The bioactivity data (GC-EAD) of the extract 2 differed from those reported for the Tifton, GA, USA population. These data suggest polymorphism in relation to the insect populations found in Brazil and in the USA. The possibility of the existence of an E. lignosellus sub-species cannot be ruled out.
650 $aacetates
650 $aInseto
700 1 $aSILVA, A. A. da
700 1 $aLIMA, E. R.
700 1 $aVIANA, P.
773 $tJournal of Separation Science, Weinheim$gv. 28, n. 3, p. 281-285, 2005.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/01/2011 |
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Data da última atualização: |
19/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, M. F. e; MIROLI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; FREITAS ASTUA, J. de; MACHADO, M. A. |
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Afiliação: |
Marina França e Silva, CNPDIA; Débora Marcondes Bastos Pereira Miroli, CNPDIA; Marcelo C. B. Cardinali, CNPDIA; Yvonne P. Mascarenhas, USP; Polyanna kelly Martins, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Marcos Antonio Machado, APTA. |
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Título: |
Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
120_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
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Conteúdo: |
Several diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection. MenosSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early ci... Mostrar Tudo |
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Palavras-Chave: |
Candidatus Liberibater asiaticus; Plant disease. |
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Thesagro: |
Bactéria; Doença de Planta; Fruta Cítrica; Greening. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02492nam a2200265 a 4500
001 1872725
005 2011-01-19
008 2010 bl uuuu u00u1 u #d
100 1 $aSILVA, M. F. e
245 $aApplication of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease.
260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010
500 $a120_PS2 Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection.
650 $aBactéria
650 $aDoença de Planta
650 $aFruta Cítrica
650 $aGreening
653 $aCandidatus Liberibater asiaticus
653 $aPlant disease
700 1 $aMIROLI, D. M. B. P.
700 1 $aCARDINALI, M. C. B.
700 1 $aMASCARENHAS, Y. P.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS ASTUA, J. de
700 1 $aMACHADO, M. A.
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